Journal: Bioactive Materials

Article Title: Preferentially released miR-122 from cyclodextrin-based star copolymer nanoparticle enhances hepatoma chemotherapy by apoptosis induction and cytotoxics efflux inhibition

doi: 10.1016/j.bioactmat.2021.03.026

Figure Lengend Snippet: In vivo therapeutic effects of sCDP/DOX/miR-122. (a) Scheme of tumor inoculation and systemic injection ( i.v. via the tail vein) of saline, free DOX, sCDP/DOX and sCDP/DOX/miR-122 in HepG2 tumor-bearing nude mice. Dose: DOX of 2.0 mg kg −1 and miR-122 of 50.0 nmol kg −1 . (b) Tumor growth curves of tumor-bearing mice after various treatments ( n = 6). (c) Image tumors removed from the mice at the end point of the study. (d) Immunohistochemistry images of tumor sections stained by anti-Ki67 antibody and TUNEL assay. The brown color represents staining of positive cells. (e) HE stained images of various tissues from the mice. * P < 0.05 and ** P < 0.01 were used as significant difference between these two groups.

Article Snippet: The proliferated cells in tumor tissue were stained by rabbit polyclonal antibody against Ki67 (ab15580, Abcam, Cambrige, MA, USA).

Techniques: In Vivo, Injection, Immunohistochemistry, Staining, TUNEL Assay

Journal: ecancermedicalscience

Article Title: Expression of the metastasis suppressor BRMS1 in uveal melanoma

doi: 10.3332/ecancer.2014.410

Figure Lengend Snippet: Expression levels of BRMS1 mRNA in four human UM cell lines, determined by RT-PCR. All four cell lines express BRMS1 in the mRNA level. The relative expression across cell lines is similar.

Article Snippet: Slides were incubated with the mouse antihuman monoclonal antibody against BRMS1 (M01), clone 2D4-2G11 (Abnova Corporation, Taiwan), at a dilution of 1:500 for 40 min at room temperature, followed by application of biotinylated secondary antibody (8 min, 37 oC), then an avidin/streptavidin enzyme conjugate complex (8 min, 37 oC).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: ecancermedicalscience

Article Title: Expression of the metastasis suppressor BRMS1 in uveal melanoma

doi: 10.3332/ecancer.2014.410

Figure Lengend Snippet: Western blot results revealing BRMS1 protein expression in four human UM cell lines. Immunoblotting shows a positive band at ~26.8 kDa for all cell lines. The cell lines had different BRMS1 protein levels; MKT-BR, the least metastatic of the four cell lines, had the highest protein expression.

Article Snippet: Slides were incubated with the mouse antihuman monoclonal antibody against BRMS1 (M01), clone 2D4-2G11 (Abnova Corporation, Taiwan), at a dilution of 1:500 for 40 min at room temperature, followed by application of biotinylated secondary antibody (8 min, 37 oC), then an avidin/streptavidin enzyme conjugate complex (8 min, 37 oC).

Techniques: Western Blot, Expressing

Journal: ecancermedicalscience

Article Title: Expression of the metastasis suppressor BRMS1 in uveal melanoma

doi: 10.3332/ecancer.2014.410

Figure Lengend Snippet: The immunostaining pattern of BRMS1 in primary human UM specimens. (a) UM specimen showing cytoplasmatic staining for anti-BRMS1. Macrophages at the lower left of the picture have a higher staining intensity than UM cells (×200 magnification). (b) UM specimen with nuclear staining (×400 magnification). (c) UM specimen demonstrating a nuclear–cytoplasmatic staining pattern (×400 magnification).

Article Snippet: Slides were incubated with the mouse antihuman monoclonal antibody against BRMS1 (M01), clone 2D4-2G11 (Abnova Corporation, Taiwan), at a dilution of 1:500 for 40 min at room temperature, followed by application of biotinylated secondary antibody (8 min, 37 oC), then an avidin/streptavidin enzyme conjugate complex (8 min, 37 oC).

Techniques: Immunostaining, Staining

Journal: Frontiers in Aging Neuroscience

Article Title: Subtype and Regional-Specific Neuroinflammation in Sporadic Creutzfeldt–Jakob Disease

doi: 10.3389/fnagi.2014.00198

Figure Lengend Snippet: Protein expression of immune mediators in sCJD is shown . (A) Western blot analysis of IL10, IL10RA, and IL6 in the frontal cortex and cerebellum of control, sCJD MM1, and sCJD VV2 cases. Four representative cases are shown. GAPDH immunostaining was used to normalize protein loading. Densitometry values of all the cases analyzed by western blot: control ( n = 15), sCJD MM1 ( n = 15), sCJD VV2 ( n = 15) show a significant increase in the expression of IL10, IL10RA, and IL6 in MM1 and VV2 samples. * p > 0.05, ** p > 0.005, *** p > 0.001: control compared with sCJD; # p > 0.05, ## p > 0.005 sCJD MM1 compared with sCJD VV2. AU: arbitrary units. (B) ELISA of IL6 and TNFα in the frontal cortex and cerebellum of control, sCJD MM1, and sCJD VV2 cases. Values obtained from control ( n = 10), sCJD MM1 ( n = 10), sCJD VV2 ( n = 10) reveal significant increase in the expression of IL-6 and TNF-α in frontal cortex and cerebellum in sCJD. * p > 0.05, ** p > 0.005, *** p > 0.001: control compared with sCJD; # p > 0.05 sCJD MM1 compared with sCJD VV2.

Article Snippet: For immunohistochemistry, the following antibodies were used: rabbit polyclonal antibodies against interleukin-10 (IL10, AP52181PU-N, Acris) diluted 1/1,000; interleukin-10RA (IL10RA, AP20308PU-N, Acris) diluted 1/50; interleukin-6 (IL6, ab6672, Abcam) diluted 1/100; macrophage colony stimulating factor (H300; M-CSF, sc13103 Santa Cruz) diluted 1/100; COX-2 (160107 Cayman) diluted 1/100; and mouse monoclonal antibodies against TNF-α (ab1793, Abcam) diluted 1/10.

Techniques: Expressing, Western Blot, Immunostaining, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Subtype and Regional-Specific Neuroinflammation in Sporadic Creutzfeldt–Jakob Disease

doi: 10.3389/fnagi.2014.00198

Figure Lengend Snippet: Protein expression levels and cellular distribution of inflammatory mediators in sCJD are shown . (A) Western blotting analysis of COX-2, SOD1, and C4-A in the frontal cortex and cerebellum in three representative cases each for control, sCJD MM1 and sCJD VV2 cases. GAPDH immunostaining was used to normalize total protein loading. Densitometry values of western blots result from the analysis of 15 control (Con), 15 MM1, and 15 VV2 cases. Region- and subtype-dependent significant increased expression is found in sCJD when compared to controls. * p > 0.05, ** p > 0.005, *** p > 0.001 control versus sCJD; # p > 0.05, ## p > 0.005 sCJD MM1 versus VV2. AU: arbitrary units. (B) Immunohistochemistry of cytokines and immune mediators in sCJD MM1 frontal cortex (a, c, e, g, i) and cerebellum (b, d, f, h, j); a, b: IL6; c, d: IL10; e, f: ILR10; g, h: TNF-alpha; i, j: M-CSF. Note that IL6, IL10RA, and TNF-α are expressed in glial cells, mainly microglia, whereas IL10, M-CSF, and TNFα are expressed in neurons. Paraffin sections, slightly counterstained with hematoxylin. Bar = 10 μm.

Article Snippet: For immunohistochemistry, the following antibodies were used: rabbit polyclonal antibodies against interleukin-10 (IL10, AP52181PU-N, Acris) diluted 1/1,000; interleukin-10RA (IL10RA, AP20308PU-N, Acris) diluted 1/50; interleukin-6 (IL6, ab6672, Abcam) diluted 1/100; macrophage colony stimulating factor (H300; M-CSF, sc13103 Santa Cruz) diluted 1/100; COX-2 (160107 Cayman) diluted 1/100; and mouse monoclonal antibodies against TNF-α (ab1793, Abcam) diluted 1/10.

Techniques: Expressing, Western Blot, Immunostaining, Immunohistochemistry

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Mao-to Prolongs the Survival of and Reduces TNF-α Expression in Mice with Viral Myocarditis

doi: 10.1093/ecam/nen010

Figure Lengend Snippet: Myofiber diameter, cardiac histopathologic scores and grading of cardiac expression of TNF-α protein in murine viral myocarditis. Groups as are described in Fig. 2. Grades of severity of necrosis and mononuclear cell infiltration on a scale from 1 to 4 are as follows: grade 1, lesions involving <25% of the ventricular myocardium; grade 2, lesions involving 25-50% of the myocardium; grade 3, lesions involving 50-75% of the myocardium; and grade 4, lesions involving 75-100% of the myocardium. ND; not determined, * P < 0.05 vs. group N, Δ P < 0.05 vs. group A.

Article Snippet: Next, the slides were incubated with an antibody directed against murine TNF-α (Alpha-Diagnostic International Inc., San Antonio, USA).

Techniques: Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Mao-to Prolongs the Survival of and Reduces TNF-α Expression in Mice with Viral Myocarditis

doi: 10.1093/ecam/nen010

Figure Lengend Snippet: A . Pathologic findings of the heart in mice after viral inoculation. Myocardial necrosis and immune cell infiltration was observed on day 6. However, myocardial necrosis in group C on day 6 was reduced compared with the groups A, B and D. B . Immunohistochemical findings showed cardiac expression of TNF-α in the heart. Myofibers in the group C were less positive than in the groups A, B or D. Group A: administered with 0.1 ml saline starting on day 0 to day 6, group B: administered with 0.1 ml Mao-to starting on day 0 to day 4, group C: administered with 0.1 ml Mao-to starting on day 2 to day 6, group D: administered 0.1 ml Mao-to from day 5 to day 10.

Article Snippet: Next, the slides were incubated with an antibody directed against murine TNF-α (Alpha-Diagnostic International Inc., San Antonio, USA).

Techniques: Immunohistochemical staining, Expressing, Saline

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Mao-to Prolongs the Survival of and Reduces TNF-α Expression in Mice with Viral Myocarditis

doi: 10.1093/ecam/nen010

Figure Lengend Snippet: Comparative expression of cardiac TNF-α mRNA. This expression in the group C were significantly less than in the groups A, B or D. Group A: administered with 0.1 ml saline starting on day 0 to day 6, group B: administered with 0.1 ml Mao-to starting on day 0 to day 4, group C: administered with 0.1 ml Mao-to starting on day 2 to day 6, group D: administered 0.1 ml Mao-to from day 5 to day 10.

Article Snippet: Next, the slides were incubated with an antibody directed against murine TNF-α (Alpha-Diagnostic International Inc., San Antonio, USA).

Techniques: Expressing, Saline